recombinant human growth hormone (rhgh) (Genentech inc)
Structured Review
Recombinant Human Growth Hormone (Rhgh), supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Recombinant:Article Title: Effects of separate and combined therapy with growth hormone and parathyroid hormone on lumbar vertebral bone in aged ovariectomized osteopenic rats. Article Snippet: Previous studies have demonstrated that growth hormone (GH) has a marked anabolic effect on cortical bone, and parathyroid hormone (PTH) has been shown to increase cancellous bone markedly and cortical bone to some extent in ovariectomized (ovx) rats.. Combined therapies mostly focused on combining a bone anabolic agent with an antiresorptive agent.. The following study was carried out to examine the efficacy of combined therapy with GH and PTH, two bone anabolic agents in rebuilding bone after loss due to ovariectomy in lumbar vertebrae, which contain both cortical and cancellous bones. Article Title: Charged filtration membranes and uses therefor Article Snippet: .. FIG. 10 presents the sieving of a smaller ( ̃22 kD, pI=5.2 ( Article Title: Elution-modified displacement chromatography coupled with electrospray ionization-MS: on-line detection of trace peptides at low-femtomole level in peptide digests. Article Snippet: Elution-modified displacement chromatography (EMDC) was employed to achieve peptide separations with high efficiency.. On-line ESI-MS and ESI-MS/MS measurements showed enrichment and detection of kemptide, a protein kinase A peptide substrate, at low femtomole levels when it was added as a trace marker component to a tryptic digest of bovine serum proteins or to a human growth hormone peptide digest at concentration ratios between 1:105 and 1:106.. In another EMDC separation, five peptides were detected in a mixture containing 20 fmol of human growth hormone tryptic digest mixed with the bovine serum protein digest. Article Title: Functional domains of human growth hormone necessary for the adipogenic activity of hGH/hPL chimeric molecules. Article Snippet: Human growth hormone (hGH) stimulates tissue growth (Chawla et al., 1983) and promotes differentiation of mesenchymal-derived cell lineages (Ohlsson et al., 1992; Green et al., 1985) including adipocytes (Green et al., 1985; Morikawa et al., 1982).. It also modulates metabolism of proteins, carbohydrates and lipids (Chawla et al., 1983).. Enzymatic and chemical cleavage studies showed the existence of molecular domains essential for growth hormone activities (Li and Graf, 1974; Reagan et al., 1975; Yamasaki et al., 1970). Article Title: Modulation of intestinal vitamin D receptor by ovariectomy, estrogen and growth hormone. Article Snippet: Age-related decline in intestinal calcium (Ca) absorption often occur in postmenopausal osteoporotic women.. The impaired Ca absorption can be corrected by estrogen (E2) therapy.. Growth hormone (GH) therapy has also been reported to increase intestinal absorption of calcium. other:Article Title: Investigation on fouling mechanisms for recombinant human growth hormone sterile filtration. Article Snippet: 0 During sterile filtration of recombinant human growth hormone solutions, severe membrane fouling was experienced compared to other protein preparations of significantly higher molecular weights and concentrations.. This phenomenon was attributed to rhGH aggregation/adsorption occurring in the filter pore.. To better understand this phenomenon, we examined several possible fouling mechanisms: (1) pore constriction, (2) adsorption due to nonspecific binding between protein and the membrane, (3) shear-induced adsorption, (4) hydrophobic interface-induced aggregation/adsorption. Molecular Weight:Article Title: Charged filtration membranes and uses therefor Article Snippet: .. FIG. 10 presents the sieving of a smaller ( ̃22 kD, pI=5.2 ( Purification:Article Title: Charged filtration membranes and uses therefor Article Snippet: .. FIG. 10 presents the sieving of a smaller ( ̃22 kD, pI=5.2 ( Produced:Article Title: On the structural preservation of recombinant human growth hormone in a dried film of a synthetic biodegradable polymer. Article Snippet: 0 In this work we describe the structural investigation of the model protein recombinant human growth hormone (rhGH) under conditions relevant to polymeric sustained-delivery depots, including the dried protein entrapped in a film of poly(DL-lactic-co-glycolic)acid.. At each step of the procedure, dehydration of rhGH by lyophilization, suspension in methylene chloride, and drying from that suspension, the structure of rhGH was probed noninvasively using Fourier transform infrared (FTIR) spectroscopy.. We found that the structure of rhGH was significantly changed by the dehydration process as indicated by a marked drop in the R-helix content and increase in the â-sheet content. |
